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  • 溴化十六烷基三甲銨瓊脂培養基基礎配方

    發布時間:

    2022-12-27

    作者:


    英文名稱:Cetrimide  Agar  Medium
    產品編號:11735-1
    產品規格:250g/瓶
    產品用途:用于銅綠假單胞菌的選擇性分離
     
    Formula(配方): g/L
    Pancreatic Digest of Gelatin(明膠胰酶水解        20.0g
    Potassium Sulfate(硫酸鉀)        10.0g
    Magnesium Chloride(氯化鎂)      1.4g
    Cetrimide (溴化十六烷基三甲銨)     0.3g
    Agar(瓊脂)                                  13.6g
    pH7.4±0.2(25℃)
     
    培養基的配制方法:
        稱取本品4.53g于100ml蒸餾水或去離子水中,添加1支21702(甘油),加熱煮沸至完全溶解,121℃高壓滅菌15分鐘,滅菌結束后請搖勻,以防瓊脂沉積于器皿底部而凝固。
     
       

      
     
     
     
    Cetrimide Agar Medium
    Product number: 11735-1
    Product specification: 250g/ bottle
    Application: used for selective isolation of pseudomonas aeruginosa
     
    Formula: g/L
    Pancreatic Digest of sunlight (gelatine trypsin hydrolysis 20.0g)
    Potassium Sulfate, Potassium Sulfate) 10.0 g
    Magnesium Chloride, Magnesium Chloride) 1.4 g
    Cetrimide (hexadecyl trimethylammonium bromide) 0.3g
    Agar(Agar) 13.6g
    PH7.4 slightly + / - 0.2 (25 ℃)
     
     
    Preparation method:
    Said the 4.53 g amount of this product in 100 ml of distilled water or deionized water, add 1-21702 (glycerin), heat to boil until completely dissolved, 121 ℃ high pressure sterilization for 15 minutes, please shake after sterilization, to prevent AGAR deposited on the bottom of the vessel and solidification.
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