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  • 志賀氏菌增菌肉湯基礎培養基配方

    發布時間:

    2022-12-27

    作者:

    日水生物


    志賀氏菌增菌肉湯配制方法:
       稱取本品31.5g于1L蒸餾水或去離子水中(可按比例增加或減少配制量),加熱煮沸至完全溶解,分裝,121℃高壓滅菌15分鐘,冷卻至50-55℃,每225ml中添加1支20410,備用。
     
    Formula(志賀氏菌增菌肉湯配方): g/L
     
    Tryptone (胰蛋白胨)                            20.0g
    Dextrose(葡萄糖)                                         1.0g
    Dipotassium phosphate 
    (磷酸氫二鉀)                                                 2.0g
    Monopotassium phosphate
    (磷酸二氫鉀)                                                2.0g
    Sodium Chloride(氯化鈉)                           5.0g
    Tween 80(吐溫80)                                     1.5ml
    pH7.0±0.2(25℃)       
        
     
    Preparation method:
    According to take this product 31.5 g in 1 l of distilled water or deionized water (can be in proportion to increase or decrease preparation), heating boiling until completely dissolved, repackaging, 121 ℃ high pressure sterilization for 15 minutes, cooled to 50 to 55 ℃, each 225 ml, 1-20410 is added to the standby.
     
    Formula: g/L
     
    Tryptone (Tryptone) 20.0g
    Dextrose(glucose) 1.0g
    Dipotassium phosphate
    (dipotassium hydrogen phosphate) 2.0g
    Monopotassium phosphate
    (potassium dihydrogen phosphate) 2.0g
    Sodium Chloride (nacl) 5.0 g
    Tween 80(twain 80) 1.5ml
    PH7.0 + / - 0.2 (25 ℃)
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