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  • 改良平板計數瓊脂MPCA配制說明

    發布時間:

    2022-12-27

    作者:

    青島日水培養基公司


    改良平板計數瓊脂配制方法:稱取本品24.5g于1L蒸餾水或去離子水中[可按比例增加或減少配制量],加熱煮沸至完全溶解,分裝,121℃高壓滅菌15分鐘,滅菌結束后請搖勻,以防瓊脂沉積于器皿底部而凝固,備用。

    modifiedplatecount agar Formula[配方]: g/L
    Tryptone [胰蛋白胨]:5.0g
    Yeast Extract[酵母浸粉]:2.5g
    Dextrose[葡萄糖]:1.0g
    Defatted milk powder[脫脂奶粉]:1.0g
    Agar[瓊脂] :15.0g
    pH7.0±0.2[25℃]
     
    Modified plate counting agar preparation method: weigh this product 24.5g in 1L distilled water or deionized water [can increase or reduce the amount of preparation according to proportion], heat and boil until completely dissolved, pack, high pressure sterilization at 121 C for 15 minutes, shake well after sterilization, in order to prevent agar deposition in the bottom of the vessel and solidification, reserve.
    Modifiedplatecount agar Formula[formula]:g/L
    Tryptone [tryptone]:5.0g
    Yeast Extract[yeast extract powder]:2.5g
    Dextrose[glucose]:1.0g
    Defatted milk powder[skim milk powder]:1.0g
    Agar[agar]:15.0g
    PH7.0 + 0.2[25 C]
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