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  • 沙氏葡萄糖瓊脂培養基SDA氯霉素配方

    發布時間:

    2022-12-27

    作者:

    山東培養基公司日水生物


    沙氏葡萄糖瓊脂培養基SDA氯霉素配制方法:稱取本品65.1g于1L蒸餾水或去離子水中,加熱煮沸至完全溶解,分裝,121℃高壓滅菌15分鐘,滅菌結束后請搖勻,以防瓊脂沉淀于器皿底部而凝固,備用。

    Sabouraud’s Glucose Agar Medium with Chloramphenicol Formula[配方]: g/L
    Mixture of Peptic Digest of Animal Tissue
    and Pancreatic Digest of  Casein [1:1)[動物組織胃蛋白酶水解物和胰酪胨等量混合物]:10.0g
    Dextrose[葡萄糖]:40.0g
    Chloramphenicol[氯霉素]:0.1g
    Agar[瓊脂]:15.0g
    pH5.6±0.2[25℃]
     
    Sachs glucose agar medium SDA chloramphenicol preparation method: 65.1g in 1L distilled water or deionized water, heated and boiled until completely dissolved, sub-packed, high-pressure sterilization at 121 ~C for 15 minutes, after sterilization, shake well, in order to prevent agar precipitation in the bottom of the vessel and solidification, reserve.
    Sabouraud 's Glucose Agar Medium with Chloramphenicol Formula[formula]:g/L
    Mixture of Peptic Digest of Animal Tissue
    Pancreatic Digest of Casein [1:1] [Mixture of pepsin hydrolysate and peptone in animal tissues]: 10.0g
    Dextrose[glucose]:40.0g
    Chloramphenicol[chloramphenicol]:0.1g
    Agar[agar]:15.0g
    PH5.6 + 0.2[25 C]
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